Now showing items 3-22 of 38

  • Antagonistic coevolution accelerates molecular evolution 

    Paterson, Steve; Vogwill, Tom; Buckling, Angus; Benmayor, Rebecca; Spiers, Andrew J.; Thomson, Nicholas R.; Quail, Mike; Smith, Frances; Walker, Danielle; Libberton, Ben; Fenton, Andrew; Hall, Neil; Brockhurst, Michael A. (Nature Publishing Group, 2010-03-11)
    The Red Queen hypothesis proposes that coevolution of interacting species (such as hosts and parasites) should drive molecular evolution through continual natural selection for adaptation and counter-adaptation. Although ...
  • Bacterial evolution in simple microcosms 

    Spiers, Andrew J. (Nova Publishers, 2013)
    Microcosms have been used extensively to investigate ecological factors, evolutionary dynamics and the underlying molecular biology of adapting populations of bacteria in experimental evolution studies. One such experimental ...
  • Bacteriophages limit the existence conditions for conjugative plasmids 

    Harrison, Ellie; Wood, Jamie A.; Dytham, Calvin; Pitchford, Jonathan W.; Truman, Julie; Spiers, Andrew J.; Paterson, Steve; Brockhurst, Michael A. (American Society for Microbiology, 2015-06-02)
    Bacteriophages are a major cause of bacterial mortality and impose strong selection on natural bacterial populations, yet their effects on the dynamics of conjugative plasmids have rarely been tested. We combined experimental ...
  • Biofilm formation and cellulose expression among diverse environmental Pseudomonas isolates 

    Ude, Susanne; Arnold, Dawn L.; Moon, Christina D.; Timms-Wilson, Tracey; Spiers, Andrew J. (Blackwell Publishing Ltd, 2006-11)
    The ability to form biofilms is seen as an increasingly important colonization strategy among both pathogenic and environmental bacteria. A survey of 185 plant-associated, phytopathogenic, soil and river Pseudomonas isolates ...
  • Biofilm formation at the air–liquid interface by the Pseudomonas fluorescens SBW25 wrinkly spreader requires an acetylated form of cellulose 

    Spiers, Andrew J.; Bohannon, John; Gehrig, Stefanie M.; Rainey, Paul B. (Blackwell Publishing Ltd, 2003-10)
    The wrinkly spreader (WS) genotype of Pseudomonas fluorescens SBW25 colonizes the air–liquid interface of spatially structured microcosms resulting in formation of a thick biofilm. Its ability to colonize this niche is ...
  • Cellulose expression in Pseudomonas fluorescens SBW25 and other environmental Pseudomonads 

    Spiers, Andrew J.; Folorunso, Ayorinde O.; Zawadzki, Kamil; Deeni, Yusuf Y.; Koza, Anna; Moshynets, Olena (InTech, 2013-08-29)
  • Characterization of a novel air–liquid interface biofilm of Pseudomonas fluorescens SBW25 

    Koza, Anna; Hallett, Paul D.; Moon, Christina D.; Spiers, Andrew J. (Society for General Microbiology, 2009)
    Pseudomonads are able to form a variety of biofilms that colonize the air–liquid (A–L) interface of static liquid microcosms, and differ in matrix composition, strength, resilience and degrees of attachment to the microcosm ...
  • Characterizing the regulation of the Pu promoter in Acinetobacter baylyi ADP1 

    Huang, Wei E.; Singer, Andrew C.; Spiers, Andrew J.; Preston, Gail M.; Whiteley, Andrew S. (Wiley-Blackwell, 2008-07)
    Effective gene trapping and screening requires sensory and regulatory compatibility of both host and exogenous systems. The naturally competent bacterium Acinetobacter baylyi ADP1 is able to efficiently take up and integrate ...
  • Environmental modification and niche construction: developing O2 gradients drive the evolution of the Wrinkly Spreader 

    Koza, Anna; Moshynets, Olena; Otten, Wilfred; Spiers, Andrew J. (International Society for Microbial Ecology, 2011-04)
    The evolutionary success of the novel Wrinkly Spreader (WS) genotypes in diversifying Pseudomonas fluorescens SBW25 populations in static liquid microcosms has been attributed to the greater availability of O2 at the ...
  • The environmental plasmid pQBR103 alters the single-cell raman spectral profile of Pseudomonas fluorescens SBW25 

    Ude, Susanne; Bailey, Mark J.; Huang, Wei E.; Spiers, Andrew J. (Springer Verlag, 2007-04)
    Although plasmids are ubiquitous amongst phytosphere pseudomonads, the advantage and costs of plasmids for the bacterial host remain unclear. The application of single-cell Raman spectral analysis to plasmid–bacterial ...
  • Environmentally co-occurring mercury resistance plasmids are genetically and phenotypically diverse and confer variable context-dependent fitness effects 

    Hall, James P. J.; Harrison, Ellie; Lilley, Andrew K.; Paterson, Steve; Spiers, Andrew J.; Brockhurst, Michael A. (Wiley, 2015-06-25)
    Plasmids are important mobile elements that can facilitate genetic exchange and local adaptation within microbial communities. We compared the sequences of four co-occurring pQBR-family environmental mercury resistance ...
  • Evolution in a test tube: rise of the Wrinkly Spreaders 

    Green, Jennifer H.; Koza, Anna; Moshynets, Olena; Pajor, Radoslaw; Ritchie, Margaret R.; Spiers, Andrew J. (Routledge, 2011-03)
    Understanding evolutionary mechanisms is fundamental to a balanced biological education, yet practical demonstrations are rarely considered. In this paper we describe a bacterial liquid microcosm which can be used to ...
  • The evolution of biofilm-forming Wrinkly Spreaders in static microcosms and drip-fed columns selects for subtle differences in wrinkleality and fitness 

    Udall, Yvette C.; Deeni, Yusuf Y.; Hapca, Simona M.; Raikes, David; Spiers, Andrew J. (Oxford University Press, 2015-05-22)
    Experimental evolution studies are used to investigate bacterial adaptive radiation in simple microcosms. In the case of the Wrinkly Spreader, a class of biofilm-forming adaptive mutants of Pseudomonas fluorescens SBW25, ...
  • From Winogradsky's column to contemporary research using bacterial microcosms 

    Moshynets, O.; Boretska, M.; Spiers, Andrew J. (Nova Publishers, 2013)
    The fast pace of scientific research often means that contemporary investigators are unaware of critical developments resulting from the work of previous generations of scientists. We were superficially aware of Sergei ...
  • Genomic and genetic analyses of diversity and plant interactions of Pseudomonas fluorescens 

    Silby, Mark W.; Cerdeño-Tárraga, Ana M.; Vernikos, Georgios S.; Giddens, Stephen R.; Jackson, Robert W.; Preston, Gail M.; Zhang, Xue-Xian; Moon, Christina D.; Gehrig, Stefanie M.; Godfrey, Scott A. C.; Knight, Christopher G.; Malone, Jacob G.; Robinson, Zena; Spiers, Andrew J.; Harris, Simon; Challis, Gregory L.; Yaxley, Alice M; Harris, David; Seeger, Kathy; Murphy, Lee; Rutter, Simon; Squares, Rob; Quail, Michael A.; Saunders, Elizabeth; Mavromatis, Konstantinos; Brettin, Thomas S.; Bentley, Stephen D.; Hothersall, Joanne; Stephens, Elton; Thomas, Christopher M.; Parkhill, Julian; Levy, Stuart B.; Rainey, Paul B.; Thomson, Nicholas R. (BioMed Central, 2009-05)
    Background: Pseudomonas fluorescens are common soil bacteria that can improve plant health through nutrient cycling, pathogen antagonism and induction of plant defenses. The genome sequences of strains SBW25 and Pf0-1 were ...
  • Getting Wrinkly Spreaders to demonstrate evolution in schools 

    Spiers, Andrew J. (Elsevier, 2014-06)
    Understanding evolution is crucial to modern biology,but most teachers would assume that practical demonstrations of evolution in school laboratories are unfeasible. However, perhaps they have not heard of ‘evolution in a ...
  • A mechanistic explanation linking adaptive mutation, niche change, and fitness advantage for the Wrinkly Spreader 

    Spiers, Andrew J. (Hindawi Publishing Corporation, 2014-01-16)
    Experimental evolution studies have investigated adaptive radiation in static liquid microcosms using the environmental bacterium Pseudomonas fluorescens SBW25. In evolving populations a novel adaptive mutant known as the ...
  • Microbial distribution in soils: physics and scaling 

    Young, Iain M.; Crawford, John W.; Nunan, Naoise; Otten, Wilfred; Spiers, Andrew J. (Elsevier, 2008)
    In a handful of fertile soil there are billions of microorganisms and yet, even with a conservative estimate, the surface area covered by these organisms is considerably less than 1%. What does this tell us about the ...
  • The minimum information about a genome sequence (MIGS) specification 

    Field, Dawn; Garrity, George; Gray, Tanya; Morrison, Norman; Selengut, Jeremy; Sterk, Peter; Tatusova, Tatiana; Thomson, Nicholas; Allen, Michael J.; Angiuoli, Samuel V.; Ashburner, Michael; Axelrod, Nelson; Baldauf, Sandra; Ballard, Stuart; Boore, Jeffrey; Cochrane, Guy; Cole, James; Dawyndt, Peter; De Vos, Paul; dePamphilis, Claude; Edwards, Robert; Faruque, Nadeem; Feldman, Robert; Gilbert, Jack; Gilna, Paul; Glöckner, Frank Oliver; Goldstein, Philip; Guralnick, Robert; Haft, Dan; Hancock, David; Hermjakob, Henning; Hertz-Fowler, Christiane; Hugenholtz, Phil; Joint, Ian; Kagan, Leonid; Kane, Matthew; Kennedy, Jessie; Kowalchuk, George; Kottmann, Renzo; Kolker, Eugene; Kravitz, Saul; Kyrpides, Nikos; Leebens-Mack, Jim; Lewis, Suzanna E.; Li, Kelvin; Lister, Allyson L.; Lord, Phillip; Maltsev, Natalia; Markowitz, Victor; Martiny, Jennifer; Methe, Barbara; Mizrachi, Ilene; Moxon, Richard; Nelson, Karen; Parkhill, Julian; Proctor, Lita; White, Owen; Sansone, Susanna-Assunta; Spiers, Andrew J.; Stevens, Robert; Swift, Paul; Taylor, Chris; Tateno, Yoshio; Tett, Adrian; Turner, Sarah; Ussery, David; Vaughan, Bob; Ward, Naomi; Whetzel, Trish; San Gil, Ingio; Wilson, Gareth; Wipat, Anil (Nature Publishing Group, 2008-05)
    With the quantity of genomic data increasing at an exponential rate, it is imperative that these data be captured electronically, in a standard format. Standardization activities must proceed within the auspices of open-access ...