Now showing items 11-30 of 46

  • Characterization of a novel air–liquid interface biofilm of Pseudomonas fluorescens SBW25 

    Koza, Anna; Hallett, Paul D.; Moon, Christina D.; Spiers, Andrew J. (Society for General Microbiology, 2009)
    Pseudomonads are able to form a variety of biofilms that colonize the air–liquid (A–L) interface of static liquid microcosms, and differ in matrix composition, strength, resilience and degrees of attachment to the microcosm ...
  • Characterizing the regulation of the Pu promoter in Acinetobacter baylyi ADP1 

    Huang, Wei E.; Singer, Andrew C.; Spiers, Andrew J.; Preston, Gail M.; Whiteley, Andrew S. (Wiley-Blackwell, 2008-07)
    Effective gene trapping and screening requires sensory and regulatory compatibility of both host and exogenous systems. The naturally competent bacterium Acinetobacter baylyi ADP1 is able to efficiently take up and integrate ...
  • Conflicting selection alters the trajectory of molecular evolution in a tripartite bacteria-plasmid-phage interaction 

    Harrison, Ellie; Hall, James P. J.; Paterson, Steve; Spiers, Andrew J.; Brockhurst, Michael A. (Wiley, 2017-03-01)
    Bacteria engage in a complex network of ecological interactions, which includes mobile genetic elements (MGEs) such as phages and plasmids. These elements play a key role in microbial communities as vectors of horizontal ...
  • Environmental modification and niche construction: developing O2 gradients drive the evolution of the Wrinkly Spreader 

    Koza, Anna; Moshynets, Olena; Otten, Wilfred; Spiers, Andrew J. (International Society for Microbial Ecology, 2011-04)
    The evolutionary success of the novel Wrinkly Spreader (WS) genotypes in diversifying Pseudomonas fluorescens SBW25 populations in static liquid microcosms has been attributed to the greater availability of O2 at the ...
  • The environmental plasmid pQBR103 alters the single-cell raman spectral profile of Pseudomonas fluorescens SBW25 

    Ude, Susanne; Bailey, Mark J.; Huang, Wei E.; Spiers, Andrew J. (Springer Verlag, 2007-04)
    Although plasmids are ubiquitous amongst phytosphere pseudomonads, the advantage and costs of plasmids for the bacterial host remain unclear. The application of single-cell Raman spectral analysis to plasmid–bacterial ...
  • Environmentally co-occurring mercury resistance plasmids are genetically and phenotypically diverse and confer variable context-dependent fitness effects 

    Hall, James P. J.; Harrison, Ellie; Lilley, Andrew K.; Paterson, Steve; Spiers, Andrew J.; Brockhurst, Michael A. (Wiley, 2015-06-25)
    Plasmids are important mobile elements that can facilitate genetic exchange and local adaptation within microbial communities. We compared the sequences of four co-occurring pQBR-family environmental mercury resistance ...
  • Evolution in a test tube: rise of the Wrinkly Spreaders 

    Green, Jennifer H.; Koza, Anna; Moshynets, Olena; Pajor, Radoslaw; Ritchie, Margaret R.; Spiers, Andrew J. (Routledge, 2011-03)
    Understanding evolutionary mechanisms is fundamental to a balanced biological education, yet practical demonstrations are rarely considered. In this paper we describe a bacterial liquid microcosm which can be used to ...
  • The evolution of biofilm-forming Wrinkly Spreaders in static microcosms and drip-fed columns selects for subtle differences in wrinkleality and fitness 

    Udall, Yvette C.; Deeni, Yusuf Y.; Hapca, Simona M.; Raikes, David; Spiers, Andrew J. (Oxford University Press, 2015-05-22)
    Experimental evolution studies are used to investigate bacterial adaptive radiation in simple microcosms. In the case of the Wrinkly Spreader, a class of biofilm-forming adaptive mutants of Pseudomonas fluorescens SBW25, ...
  • From Winogradsky's column to contemporary research using bacterial microcosms 

    Moshynets, O.; Boretska, M.; Spiers, Andrew J. (Nova Publishers, 2013)
    The fast pace of scientific research often means that contemporary investigators are unaware of critical developments resulting from the work of previous generations of scientists. We were superficially aware of Sergei ...
  • Genomic and genetic analyses of diversity and plant interactions of Pseudomonas fluorescens 

    Silby, Mark W.; Cerdeño-Tárraga, Ana M.; Vernikos, Georgios S.; Giddens, Stephen R.; Jackson, Robert W.; Preston, Gail M.; Zhang, Xue-Xian; Moon, Christina D.; Gehrig, Stefanie M.; Godfrey, Scott A. C.; Knight, Christopher G.; Malone, Jacob G.; Robinson, Zena; Spiers, Andrew J.; Harris, Simon; Challis, Gregory L.; Yaxley, Alice M; Harris, David; Seeger, Kathy; Murphy, Lee; Rutter, Simon; Squares, Rob; Quail, Michael A.; Saunders, Elizabeth; Mavromatis, Konstantinos; Brettin, Thomas S.; Bentley, Stephen D.; Hothersall, Joanne; Stephens, Elton; Thomas, Christopher M.; Parkhill, Julian; Levy, Stuart B.; Rainey, Paul B.; Thomson, Nicholas R. (BioMed Central, 2009-05)
    Background: Pseudomonas fluorescens are common soil bacteria that can improve plant health through nutrient cycling, pathogen antagonism and induction of plant defenses. The genome sequences of strains SBW25 and Pf0-1 were ...
  • Getting Wrinkly Spreaders to demonstrate evolution in schools 

    Spiers, Andrew J. (Elsevier, 2014-06)
    Understanding evolution is crucial to modern biology,but most teachers would assume that practical demonstrations of evolution in school laboratories are unfeasible. However, perhaps they have not heard of ‘evolution in a ...
  • Limitation of biosurfactant strength produced by bacteria 

    Fechtner, Joanna; Cameron, Scott; Deeni, Yusuf Y.; Hapca, Simona M.; Kabir, Kamaluddeen; Mohammed, Ibrahim U.; Spiers, Andrew J. (Nova Science Publishers, 2017-01-01)
    Biosurfactants which reduce the surface or interfacial tension of liquids and act as emulsifiers, foaming and dispersing agents with low toxicity, are finding increasing applications in biotechnology and driving the search ...
  • A mechanistic explanation linking adaptive mutation, niche change, and fitness advantage for the Wrinkly Spreader 

    Spiers, Andrew J. (Hindawi Publishing Corporation, 2014-01-16)
    Experimental evolution studies have investigated adaptive radiation in static liquid microcosms using the environmental bacterium Pseudomonas fluorescens SBW25. In evolving populations a novel adaptive mutant known as the ...
  • Microbial distribution in soils: physics and scaling 

    Young, Iain M.; Crawford, John W.; Nunan, Naoise; Otten, Wilfred; Spiers, Andrew J. (Elsevier, 2008)
    In a handful of fertile soil there are billions of microorganisms and yet, even with a conservative estimate, the surface area covered by these organisms is considerably less than 1%. What does this tell us about the ...
  • The minimum information about a genome sequence (MIGS) specification 

    Field, Dawn; Garrity, George; Gray, Tanya; Morrison, Norman; Selengut, Jeremy; Sterk, Peter; Tatusova, Tatiana; Thomson, Nicholas; Allen, Michael J.; Angiuoli, Samuel V.; Ashburner, Michael; Axelrod, Nelson; Baldauf, Sandra; Ballard, Stuart; Boore, Jeffrey; Cochrane, Guy; Cole, James; Dawyndt, Peter; De Vos, Paul; dePamphilis, Claude; Edwards, Robert; Faruque, Nadeem; Feldman, Robert; Gilbert, Jack; Gilna, Paul; Glöckner, Frank Oliver; Goldstein, Philip; Guralnick, Robert; Haft, Dan; Hancock, David; Hermjakob, Henning; Hertz-Fowler, Christiane; Hugenholtz, Phil; Joint, Ian; Kagan, Leonid; Kane, Matthew; Kennedy, Jessie; Kowalchuk, George; Kottmann, Renzo; Kolker, Eugene; Kravitz, Saul; Kyrpides, Nikos; Leebens-Mack, Jim; Lewis, Suzanna E.; Li, Kelvin; Lister, Allyson L.; Lord, Phillip; Maltsev, Natalia; Markowitz, Victor; Martiny, Jennifer; Methe, Barbara; Mizrachi, Ilene; Moxon, Richard; Nelson, Karen; Parkhill, Julian; Proctor, Lita; White, Owen; Sansone, Susanna-Assunta; Spiers, Andrew J.; Stevens, Robert; Swift, Paul; Taylor, Chris; Tateno, Yoshio; Tett, Adrian; Turner, Sarah; Ussery, David; Vaughan, Bob; Ward, Naomi; Whetzel, Trish; San Gil, Ingio; Wilson, Gareth; Wipat, Anil (Nature Publishing Group, 2008-05)
    With the quantity of genomic data increasing at an exponential rate, it is imperative that these data be captured electronically, in a standard format. Standardization activities must proceed within the auspices of open-access ...
  • New insights into the effects of several environmental parameters on the relative fitness of a numerically dominant class of evolved niche specialist 

    Kuśmierska, Anna; Spiers, Andrew J. (Hindawi Publishing Corporation, 2016-11-24)
    Adaptive radiation in bacteria has been investigated using Wrinkly Spreaders (WS), a morphotype which colonises the air-liquid (A-L) interface of static microcosms by biofilm formation with a significant fitness advantage ...
  • Parallel compensatory evolution stabilizes plasmids across the parasitism-mutualism continuum 

    Harrison, Ellie; Guymer, David; Spiers, Andrew J.; Paterson, Steve; Brockhurst, Michael A. (Elsevier, 2015-07-16)
    Plasmids drive genomic diversity in bacteria via horizontal gene transfer [1 and 2]; nevertheless, explaining their survival in bacterial populations is challenging [3]. Theory predicts that irrespective of their net fitness ...
  • The phytosphere of Brassica napus L. as a niche for Pseudomonas fluorescens SBW25. 

    Moshynets, O. V.; Shpylova, S. P.; Spiers, Andrew J.; Kosakivska, I. V. (National Academy of Sciences of Ukraine, 2010)
  • Plasmid carriage can limit bacteria–phage coevolution 

    Harrison, Ellie; Truman, Julie; Wright, Rosanna; Spiers, Andrew J.; Paterson, Steve; Brockhurst, Michael A. (The Royal Society, 2015-08-12)
    Coevolution with bacteriophages is a major selective force shaping bacterial populations and communities. A variety of both environmental and genetic factors has been shown to influence the mode and tempo of bacteria–phage ...