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Title: Elasticity of human embryonic stem cells as determined by atomic force microscopy
Authors: Kiss, Robert
Bock, Henry
Pells, Steve
Canetta, Elisabetta
Adya, Ashok K.
Moore, Andrew J.
De Sousa, Paul
Willoughby, Nicholas A.
Affiliation: University of Abertay Dundee. School of Contemporary Sciences
Keywords: Atomic force microscopy
Cellular biophysics
Tissue engineering
Young's modulus
Issue Date: Oct-2011
Publisher: American Society of Mechanical Engineers
Type: Journal Article
Refereed: peer-reviewed
Rights: Published version (c)American Society of Mechanical Engineers, available from http://dx.doi.org/10.1115/1.4005286
Citation: Kiss, R., et al. 2011. Elasticity of human embryonic stem cells as determined by atomic force microscopy. Journal of Biomechanical Engineering. 133(10): 101009. Available from http://dx.doi.org/10.1115/1.4005286
Abstract: The expansive growth and differentiation potential of human embryonic stem cells (hESCs) make them a promising source of cells for regenerative medicine. However, this promise is off set by the propensity for spontaneous or uncontrolled differentiation to result in heterogeneous cell populations. Cell elasticity has recently been shown to characterize particular cell phenotypes, with undifferentiated and differentiated cells sometimes showing significant differences in their elasticities. In this study, we determined the Young's modulus of hESCs by atomic force microscopy using a pyramidal tip. Using this method we are able to take point measurements of elasticity at multiple locations on a single cell, allowing local variations due to cell structure to be identified. We found considerable differences in the elasticity of the analyzed hESCs, reflected by a broad range of Young's modulus (0.05-10 kPa). This surprisingly high variation suggests that elasticity could serve as the basis of a simple and efficient large scale purification/separation technique to discriminate subpopulations of hESCs.
URI: http://hdl.handle.net/10373/1168
ISSN: 0148-0731
Appears in Collections:Science Engineering & Technology Collection

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