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Please use this identifier to cite or link to this item: http://hdl.handle.net/10373/1169

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Title: Effects of the CDK-inhibitor CYC202 on p38 MAPK, ERK1/2 and c-Myc activities in papillomavirus type 16 E6- and E7-transformed human keratinocytes
Authors: Atanasova, Ganka N.
Isaeva, Antonia R.
Zhelev, Nikolai Z.
Poumay, Yves
Mitev, Vanyo I.
Affiliation: University of Abertay Dundee. Scottish Informatics, Mathematics, Biology and Statistics Centre
Keywords: Keratinocytes
HPV16 E6 and E7 genes
MAP kinases
Issue Date: Oct-2007
Publisher: Spandidos Publications
Type: Journal Article
Refereed: peer-reviewed
Rights: This is the published version of this article. Reproduced with permission from the publisher. Published version (c)Spandidos Publications, available from http://www.spandidos-publications.com/or/18/4/999
Citation: Atanasova, G.N., et al. 2007. Effects of the CDK-inhibitor CYC202 on p38 MAPK, ERK1/2 and c-Myc activities in papillomavirus type 16 E6- and E7-transformed human keratinocytes. Oncology Reports. 18(4): pp.999-1005. Available from http://www.spandidos-publications.com/or/18/4/999
Abstract: In the present study, we have investigated the effect of the chemical CDK-inhibitor CYC202 on E6 and E7-transformed keratinocytes, in which the function of the cellular cell cycle inhibitor p21Cip1 is abrogated by the viral genes. The cyto-toxicity and the inhibition of the cell growth were analysed by MTT assay and analysis of DNA synthesis respectively. The effect on some signalling molecules was tested by Western blot analysis. CYC202 effectively inhibited the proliferation of E6 and E7 keratinocytes in a dose-dependent manner. Treatment with CYC202 strongly increased the activity of p38 MAP kinase. Furthermore, it inhibited ERK1/2 at the highest concentration used and had no effect on the activity of JNK1/2. CYC202 also increased the phosphorylation of HSP27 and decreased the phosphorylation and DNA-binding activity of the transcriptional regulator c-Myc, in correlation with the corresponding upstream kinases p38 MAPK and ERK1/2. Our results provide additional data for the anti-proliferative actions and potency of the chemical CDK-inhibitor CYC202.
URI: http://hdl.handle.net/10373/1169
ISSN: 1021-335X
Appears in Collections:SIMBIOS Collection
Science Engineering & Technology Collection

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