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Please use this identifier to cite or link to this item: http://hdl.handle.net/10373/1187

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Title: The small Rho GTPase Rac1 controls normal human dermal fibroblasts proliferation with phosphorylation of the oncoprotein c-myc
Authors: Nikolova, Ekaterina
Mitev, Vanio
Zhelev, Nikolai Z.
Deroanne, Christophe F.
Poumay, Yves
Affiliation: University of Abertay Dundee. Scottish Informatics, Mathematics, Biology and Statistics Centre
Keywords: Normal human skin fibroblasts
Rac1
Phospho-ERK1/2
Phospho-p38
Phospho-c-myc
Proliferation
Issue Date: Aug-2007
Publisher: Elsevier
Type: Journal Article
Refereed: peer-reviewed
Rights: Published version (c)Elsevier, available from http://dx.doi.org/10.1016/j.bbrc.2007.05.214
Citation: Nikolova, E., et al. 2007. The small Rho GTPase Rac1 controls normal human dermal fibroblasts proliferation with phosphorylation of the oncoprotein c-myc. Biochemical and Biophysical Research Communications. 359(3): pp.834-839. Available from http://dx.doi.org/10.1016/j.bbrc.2007.05.214
Abstract: Proliferation of dermal fibroblasts is crucial for the maintenance of skin. The small Rho GTPase, Rac1, has been identified as a key transducer of proliferative signals in various cell types, but in normal human dermal fibroblasts its significance to cell growth control has not been studied. In this study, we applied the method of RNA interference to suppress endogenous Rac1 expression and examined the consequences on human skin fibroblasts. Rac1 knock-down resulted in inhibition of DNA synthesis. This effect was not mediated by inhibition of the central transducer of proliferative stimuli, ERK1/2 or by activation of the pro-apoptotic p38. Rather, as a consequence of the suppressed Rac1 expression we observed a significant decrease in phosphorylation of c-myc, revealing for the first time that in human fibroblasts Rac1 exerts control on proliferation through c-myc phosphorylation. Thus Rac1 activates proliferation of normal fibroblasts through stimulation of c-myc phosphorylation without affecting ERK1/2 activity.
URI: http://hdl.handle.net/10373/1187
ISSN: 0006-291X
Appears in Collections:SIMBIOS Collection
Science Engineering & Technology Collection

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