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Please use this identifier to cite or link to this item: http://hdl.handle.net/10373/1284

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Title: Transparent soil for imaging the rhizosphere
Authors: Downie, Helen
Holden, Nicola
Otten, Wilfred
Spiers, Andrew J.
Valentine, Tracy A.
Dupuy, Lionel X.
Affiliation: University of Abertay Dundee. Scottish Informatics, Mathematics, Biology and Statistics Centre
Keywords: Rhizosphere
Issue Date: Sep-2012
Publisher: Public Library of Science
Type: Journal Article
Refereed: peer-reviewed
Rights: This is the published version of this article. Reproduced with permission from the publisher. Published version (c)H. Downie, N. Holden, W. Otten, A.J. Spiers, T.A. Valentine, L.X. Dupuy, available from http://dx.doi.org/10.1371/journal.pone.0044276
Citation: Downie, H., et al. 2012. Transparent soil for imaging the rhizosphere. PLoS ONE. 7(9): e44276. Available from http://dx.doi.org/10.1371/journal.pone.0044276
Abstract: Understanding of soil processes is essential for addressing the global issues of food security, disease transmission and climate change. However, techniques for observing soil biology are lacking. We present a heterogeneous, porous, transparent substrate for in situ 3D imaging of living plants and root-associated microorganisms using particles of the transparent polymer, Nafion, and a solution with matching optical properties. Minerals and fluorescent dyes were adsorbed onto the Nafion particles for nutrient supply and imaging of pore size and geometry. Plant growth in transparent soil was similar to that in soil. We imaged colonization of lettuce roots by the human bacterial pathogen Escherichia coli O157:H7 showing micro-colony development. Micro-colonies may contribute to bacterial survival in soil. Transparent soil has applications in root biology, crop genetics and soil microbiology.
URI: http://hdl.handle.net/10373/1284
ISSN: 1932-6203
Appears in Collections:SIMBIOS Collection

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