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Please use this identifier to cite or link to this item: http://hdl.handle.net/10373/198

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Title: The Pseudomonas fluorescens SBW25 wrinkly spreader biofilm requires attachment factor, cellulose fibre and LPS interactions to maintain strength and integrity
Authors: Spiers, Andrew J.
Rainey, Paul B.
Affiliation: University of Abertay Dundee. Scottish Informatics, Mathematics, Biology and Statistics Centre
Keywords: Pseudomonas fluorescens
Biofilms
Cellulose
Issue Date: 2005
Publisher: Society for General Microbiology
Type: Journal Article
Refereed: peer-reviewed
Rights: Published version (c)Society for General Microbiology, available from DOI: 10.1099/mic.0.27984-0
Citation: Spiers, A. J. and Rainey, P. B. 2005. The Pseudomonas fluorescens SBW25 wrinkly spreader biofilm requires attachment factor, cellulose fibre and LPS interactions to maintain strength and integrity. Microbiology. 151(2005): pp.2829-2839. [Online] Available from: DOI: 10.1099/mic.0.27984-0
Abstract: The wrinkly spreader (WS) isolate of Pseudomonas fluorescens SBW25 forms a substantial biofilm at the air–liquid interface. The biofilm is composed of an extracellular partially acetylated cellulose-fibre matrix, and previous mutagenesis of WS with mini-Tn5 had identified both the regulatory and cellulose-biosynthetic operons. One uncharacterized WS mutant, WS-5, still expressed cellulose but produced very weak biofilms. In this work, the mini-Tn5 insertion site in WS-5 has been identified as being immediately upstream of the tol-pal operon. Like Tol-Pal mutants of other Gram-negative bacteria, WS-5 showed a ‘leaky-membrane’ phenotype, including the serendipitous ability to utilize sucrose, increased uptake of the hydrophilic dye propidium iodide, and the loss of lipopolysaccharide (LPS) expression. WS-5 cells were altered in relative hydrophobicity, and showed poorer recruitment and maintenance in the biofilm than WS. The WS-5 biofilm was also less sensitive to chemical interference during development. However, growth rate, cellulose expression and attachment were not significantly different between WS and WS-5. Finally, WS-5 biofilms could be partially complemented with WS-4, a biofilm- and attachment-deficient mutant that expressed LPS, resulting in a mixed biofilm with significantly increased strength. These findings show that a major component of the WS air–liquid biofilm strength results from the interactions between LPS and the cellulose matrix of the biofilm – and that in the WS biofilm, cellulose fibres, attachment factor and LPS are required for biofilm development, strength and integrity.
URI: http://hdl.handle.net/10373/198
ISSN: 1465-2080
Appears in Collections:SIMBIOS Collection

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