Abertay Research Collections >
School of Science, Engineering & Technology >
Science Engineering & Technology Collection >
Please use this identifier to cite or link to this item:
|Title: ||Physiological and transcriptional responses of Saccharomyces cerevisiae to zinc limitation in chemostat cultures|
|Authors: ||Nicola, Raffaele De|
Hazelwood, Lucie A.
Hulster, Erik A. F. De
Walsh, Michael C.
Knijnenburg, Theo A.
Reinders, Marcel J. T.
Walker, Graeme M.
Pronk, Jack T.
|Affiliation: ||University of Abertay Dundee. School of Contemporary Sciences|
|Keywords: ||Saccharomyces cerevisiae|
|Issue Date: ||Dec-2007|
|Publisher: ||American Society for Microbiology|
|Type: ||Journal Article|
|Rights: ||Published version (c)American Society for Microbiology, available from DOI: 10.1128/AEM.01445-07|
|Citation: ||Nicola, R. D., et al. 2007. Physiological and transcriptional responses of Saccharomyces cerevisiae to zinc limitation in chemostat cultures. Applied and Environmental Microbiology. 73(23): pp.7680-7692. [Online] Available from: DOI: 10.1128/AEM.01445-07|
|Abstract: ||Transcriptional responses of the yeast Saccharomyces cerevisiae to Zn availability were investigated at a fixed specific growth rate under limiting and abundant Zn concentrations in chemostat culture. To investigate the context dependency of this transcriptional response and eliminate growth rate-dependent variations in transcription, yeast was grown under several chemostat regimens, resulting in various carbon (glucose), nitrogen (ammonium), zinc, and oxygen supplies. A robust set of genes that responded consistently to Zn limitation was identified, and the set enabled the definition of the Zn-specific Zap1p regulon, comprised of 26 genes and characterized by a broader zinc-responsive element consensus (MHHAACCBYNMRGGT) than so far described. Most surprising was the Zn-dependent regulation of genes involved in storage carbohydrate metabolism. Their concerted down-regulation was physiologically relevant as revealed by a substantial decrease in glycogen and trehalose cellular content under Zn limitation. An unexpectedly large number of genes were synergistically or antagonistically regulated by oxygen and Zn availability. This combinatorial regulation suggested a more prominent involvement of Zn in mitochondrial biogenesis and function than hitherto identified.|
|Appears in Collections:||Science Engineering & Technology Collection|
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.