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Please use this identifier to cite or link to this item: http://hdl.handle.net/10373/318

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Title: Atomic force microscopic study of the effects of ethanol on yeast cell surface morphology
Authors: Canetta, Elisabetta
Adya, Ashok K.
Walker, Graeme M.
Affiliation: University of Abertay Dundee. School of Contemporary Sciences
Keywords: AFM
Atomic force microscopy
Cell morphology
Cell physiology
Ethanol stress
Issue Date: Feb-2006
Publisher: Blackwell Publishing
Type: Journal Article
Refereed: peer-reviewed
Rights: Published version (c)Blackwell Publishing, available from DOI: 10.1111/j.1574-6968.2005.00089.x. The definitive version is available at www.blackwell-synergy.com
Citation: Canetta, E., Adya, A. K. and Walker, G. M. 2006. Atomic force microscopic study of the effects of ethanol on yeast cell surface morphology. FEMS Microbiology Letters. 255(2): pp.308-315. [Online] Available from: DOI: 10.1111/j.1574-6968.2005.00089.x
Abstract: The detrimental effects of ethanol toxicity on the cell surface morphology of Saccharomyces cerevisiae (strain NCYC 1681) and Schizosaccharomyces pombe (strain DVPB 1354) were investigated using an atomic force microscope (AFM). In combination with culture viability and mean cell volume measurements AFM studies allowed us to relate the cell surface morphological changes, observed on nanometer lateral resolution, with the cellular stress physiology. Exposing yeasts to increasing stressful concentrations of ethanol led to decreased cell viabilities and mean cell volumes. Together with the roughness and bearing volume analyses of the AFM images, the results provided novel insight into the relative ethanol tolerance of S. cerevisiae and Sc. pombe.
URI: http://hdl.handle.net/10373/318
ISSN: 0378-1097
Appears in Collections:Science Engineering & Technology Collection

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