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Please use this identifier to cite or link to this item: http://hdl.handle.net/10373/322

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Title: Nanoscopic morphological changes in yeast cell surfaces caused by oxidative stress: an atomic force microscopic study
Authors: Canetta, Elisabetta
Walker, Graeme M.
Adya, Ashok K.
Affiliation: University of Abertay Dundee. School of Contemporary Sciences
Keywords: Atomic force microscopy (AFM)
Yeast morphology
Yeasts physiology
Oxidative stress
Saccharomyces cerevisiae
Schizosaccharomyces pombe
Premature ageing of cells
Issue Date: 2009
Publisher: Korean Society for Microbiology and Biotechnology
Type: Journal Article
Refereed: peer-reviewed
Rights: Published version (c)Korean Society for Microbiology and Biotechnology, available from 10.4014/jmb.0809.515
Citation: Canetta, E., Walker, G. M. and Adya, A. K. 2009. Nanoscopic morphological changes in yeast cell surfaces caused by oxidative stress: an atomic force microscopic study. Korean Journal of Microbiology and Biotechnology. 19(6): pp.547-555. Available from: DOI: 10.4014/jmb.0809.515
Abstract: Nanoscopic changes in the cell surface morphology of the yeasts Saccharomyces cerevisiae (strain NCYC 1681) and Schizosaccharomyces pombe (strain DVPB 1354), due to their exposure to varying concentrations of hydrogen peroxide (oxidative stress), were investigated using an atomic force microscope (AFM). Increasing hydrogen peroxide concentration led to a decrease in cell viabilities and mean cell volumes, and an increase in the surface roughness of the yeasts. In addition, AFM studies revealed that oxidative stress caused cell compression in both S. cerevisiae and Schiz. pombe cells and an increase in the number of aged yeasts. These results confirmed the importance and usefulness of AFM in investigating the morphology of stressed microbial cells at the nanoscale. The results also provided novel information on the relative oxidative stress tolerance of S. cerevisiae and Schiz. pombe.
URI: http://hdl.handle.net/10373/322
ISSN: 1598-642X
Appears in Collections:Science Engineering & Technology Collection

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