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Please use this identifier to cite or link to this item: http://hdl.handle.net/10373/391

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Title: Bioconversion of brewer’s spent grains to bioethanol
Authors: White, Jane S.
Yohannan, Biju K.
Walker, Graeme M.
Affiliation: University of Abertay Dundee. School of Contemporary Sciences
Keywords: Spent grains
Bioethanol
Lignocellulose
Pichia stipitis
Kluyveromyces marxianus
Issue Date: Nov-2008
Publisher: Wiley-Blackwell
Type: Journal Article
Refereed: peer-reviewed
Rights: Published version (c)Wiley-Blackwell, available from DOI: 10.1111/j.1567-1364.2008.00390.x. The definitive version is available at www3.interscience.wiley.com
Citation: White, J.S., Yohannan, B.K. and Walker, G.M. 2008. Bioconversion of brewer’s spent grains to bioethanol. FEMS Yeast Research. 8(7): pp.1175 - 1184. Available at DOI: 10.1111/j.1567-1364.2008.00390.x
Abstract: Spent grains (SG), the residue remaining after extraction of wort, are a major by-product of brewing. This lignocelluose-rich biomass may provide a source of sugars for fuel ethanol fermentations. Dilute acid and enzyme treatments were developed to convert the hemicellulose and cellulose fractions to glucose, xylose and arabinose. Pretreatment of dried, milled grains with 0.16 N HNO3 at 121 °C for 15 min was chosen as the most suitable method for solubilizing grains before enzymatic digestion with cellulase and hemicellulase preparations. Solids loading concentrations (10%, 15% and 20% w/v) were compared and reducing sugar between 40 and 48 g (100 g SG)−1 was extracted. Hydrolysate, prepared from 20% SG, pretreated with 0.16 N HNO3, partially neutralized to pH 5–6 and digested with enzymes for 18 h, contained 27 g L−1 glucose, 16.7 g L−1 xylose and 11.9 g L−1 arabinose. Fermentation of this hydrolysate for 48 h by Pichia stipitis and Kluyveromyces marxianus resulted in 8.3 and 5.9 g L−1 ethanol corresponding to ethanol conversion yields of 0.32 and 0.23 g ethanol (g substrate)−1, respectively. Substrate utilization efficiency was less when compared with glucose/xylose mixtures in synthetic media, suggesting that yeast inhibitory compounds derived from SG were present in the hydrolysate.
URI: http://hdl.handle.net/10373/391
ISSN: 1567-1356
Appears in Collections:Science Engineering & Technology Collection

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