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Please use this identifier to cite or link to this item: http://hdl.handle.net/10373/398

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Title: Influence of magnesium ions on heat shock and ethanol stress responses of Saccharomyces cerevisiae
Authors: Birch, R. M.
Walker, Graeme M.
Affiliation: University of Abertay Dundee. School of Contemporary Sciences.
Keywords: Yeast
Stress
Heat shock
Ethanol stress
Magnesium
Issue Date: Jun-2000
Publisher: Elsevier Science
Type: Journal Article
Refereed: peer-reviewed
Rights: Published version (c)Elsevier, available at doi: 10.1016/S0141-0229(00)00159-9
Citation: Birch, R.M., Walker, G.M. 2000. Influence of magnesium ions on heat shock and ethanol stress responses of Saccharomyces cerevisiae. Enzyme and Microbial Technology. 26(9/10): pp.678-687. Available from doi: 10.1016/S0141-0229(00)00159-9
Abstract: This study has highlighted the role of magnesium ions in the amelioration of the detrimental effects of ethanol toxicity and temperature shock in a winemaking strain of Saccharomyces cerevisiae. Specifically, results based on measurements of cellular viability and heat shock protein synthesis together with scanning electron microscopy have shown that, by increasing the bioavailability of magnesium ions, physiological protection is conferred on yeast cells. Elevating magnesium levels in the growth medium from 2 to 20 mM results in repression of certain heat shock proteins following a typical heat shock regime (30-42 degrees C shift). Seed inocula cultures prepropagated in elevated levels of magnesium (i.e. 'preconditioned') also conferred thermotolerance on cells and repressed the biosynthesis of heat shock proteins. Similar results were observed in response to ethanol stress. Extra- and intracellular magnesium may both act in the physiological stress protection of yeast cells and this approach offers potential benefits in alcoholic fermentation processes. The working hypothesis based on our findings is that magnesium protects yeast cells by preventing increases in cell membrane permeability elicited by ethanol and temperature-induced stress.
URI: http://hdl.handle.net/10373/398
ISSN: 0141-0229
Appears in Collections:Science Engineering & Technology Collection

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