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Please use this identifier to cite or link to this item: http://hdl.handle.net/10373/437

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Title: Emulsifying properties of a glycoprotein extract produced by a marine Flexibacter species strain TG382
Authors: Gutierrez, Tony
Leo, Vincent V.
Walker, Graeme M.
Green, David H.
Affiliation: University of Abertay Dundee. School of Contemporary Sciences
Keywords: Flexibacter
Emulsifier
Exopolysaccharide
Glycoprotein
Hexadecane
Issue Date: Jul-2009
Publisher: Elsevier
Type: Journal Article
Refereed: peer-reviewed
Rights: Published version (c)2009 Elsevier Inc., is available from http://dx.doi.org/10.1016/j.enzmictec.2009.04.001
Citation: Gutierrez, T. et al. 2009. Emulsifying properties of a glycoprotein extract produced by a marine Flexibacter species strain TG382. Enzyme and Microbial Technology. 45(1). pp:53-57. DOI: 10.1016/j.enzmictec.2009.04.001
Abstract: We report, for the first time, on the production of an emulsifying polymer produced by a Flexibacter species (designated strain TG382). This polymer, E-382, was produced extracellularly during growth of the organism in a marine broth amended with glucose. After cold ethanol precipitation, extensive dialysis and lyophilization, a chemical analysis of the resultant dried polymer revealed it to be a glycoprotein composed of 10.9% protein, 23.3% carbohydrate and a 5.5% uronic acid content. At relatively low concentrations (0.02%, w/v), E-382 was found to form oil-in-water emulsions against hydrocarbon and food oils under neutral pH and acidic conditions. The most stable emulsions were formed against the oils sunflower, vegetable and ground nut under neutral pH conditions. Aqueous solutions of the polymer were viscous, and its reduced viscosity (╬Ěred) was determined to be 0.54 m3/kg. Although proteins and uronic acids may possess surface-active properties, the viscosifying effect of this polymer, which is a typical feature of some commercial hydrocolloids, is more likely to confer its high emulsion-stabilizing qualities.
URI: http://hdl.handle.net/10373/437
ISSN: 0141-0229
Appears in Collections:Science Engineering & Technology Collection

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