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|Title: ||The effect of subminimal inhibitory concentrations of antibiotics on virulence factors expressed by Staphylococcus aureus biofilms|
|Authors: ||Haddadin, R. N. S.|
Al-Adham, I. S. I.
Buultjens, T. E. J.
Collier, Phillip J.
|Affiliation: ||University of Abertay Dundee. School of Contemporary Sciences|
|Issue Date: ||Apr-2010|
|Type: ||Journal Article|
|Rights: ||Published version (c)Wiley-Blackwell, available from DOI: 10.1111/j.1365-2672.2009.04529.x. The definitive version is available at www3.interscience.wiley.com|
|Citation: ||Haddadin R.N.S., et al. 2010. The effect of subminimal inhibitory concentrations of antibiotics on virulence factors expressed by Staphylococcus aureus biofilms. Journal of Applied Microbiology. 108(4): pp.1281-1291. Available from DOI: 10.1111/j.1365-2672.2009.04529.x|
|Abstract: ||Aims: The effect of subminimal inhibitory concentrations (sub-MICs) of
cefalexin, ciprofloxacin and roxithromycin was investigated on some virulence
factors [e.g. coagulase, Toxic Shock Syndrome Toxin 1 (TSST-1) and biofilm
formation] expressed by Staphylococcus aureus biofilms.
Methods and Results: Biofilms were grown with and without the presence of
1 ⁄ 16 MIC of antibiotics on Sorbarod filters. Eluate supernatants were collected,
and coagulase and TSST-1 production were evaluated. Coagulase production
was reduced in eluates exposed to roxithromycin when compared to control,
while TSST-1 production was reduced in biofilms exposed to cefalexin and to
a lesser extent, ciprofloxacin. In addition, the ability of Staph. aureus to
produce biofilm in microtitre plates in the presence of sub-MIC antibiotics
indicated that cefalexin induced biofilm formation at a wide range of sub-
MICs. TSST-1 produced from the challenged and control biofilms was purified,
and its proliferative activity was studied on single cell suspension of mouse
splenocytes using MTS⁄PMS assay. No significant difference in the activity
between the treated toxin and the control has been observed.
Conclusions: Antibiotics at sub-MIC levels interfere with bacterial biofilm virulence
expression depending on the type and concentration of antibiotic used.
Significance and Impact of the Study: The establishment of sub-MICs of antibiotics
in clinical situations may result in altered virulence states in pathogenic
|Appears in Collections:||Science Engineering & Technology Collection|
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