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Please use this identifier to cite or link to this item: http://hdl.handle.net/10373/461

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Title: The effect of subminimal inhibitory concentrations of antibiotics on virulence factors expressed by Staphylococcus aureus biofilms
Authors: Haddadin, R. N. S.
Saleh, S.
Al-Adham, I. S. I.
Buultjens, T. E. J.
Collier, Phillip J.
Affiliation: University of Abertay Dundee. School of Contemporary Sciences
Keywords: Biofilm
Coagulase
Splenocytes
Subminimal
TSST
Virulence
Issue Date: Apr-2010
Publisher: Wiley-Blackwell
Type: Journal Article
Refereed: peer-reviewed
Rights: Published version (c)Wiley-Blackwell, available from DOI: 10.1111/j.1365-2672.2009.04529.x. The definitive version is available at www3.interscience.wiley.com
Citation: Haddadin R.N.S., et al. 2010. The effect of subminimal inhibitory concentrations of antibiotics on virulence factors expressed by Staphylococcus aureus biofilms. Journal of Applied Microbiology. 108(4): pp.1281-1291. Available from DOI: 10.1111/j.1365-2672.2009.04529.x
Abstract: Aims: The effect of subminimal inhibitory concentrations (sub-MICs) of cefalexin, ciprofloxacin and roxithromycin was investigated on some virulence factors [e.g. coagulase, Toxic Shock Syndrome Toxin 1 (TSST-1) and biofilm formation] expressed by Staphylococcus aureus biofilms. Methods and Results: Biofilms were grown with and without the presence of 1 ⁄ 16 MIC of antibiotics on Sorbarod filters. Eluate supernatants were collected, and coagulase and TSST-1 production were evaluated. Coagulase production was reduced in eluates exposed to roxithromycin when compared to control, while TSST-1 production was reduced in biofilms exposed to cefalexin and to a lesser extent, ciprofloxacin. In addition, the ability of Staph. aureus to produce biofilm in microtitre plates in the presence of sub-MIC antibiotics indicated that cefalexin induced biofilm formation at a wide range of sub- MICs. TSST-1 produced from the challenged and control biofilms was purified, and its proliferative activity was studied on single cell suspension of mouse splenocytes using MTS⁄PMS assay. No significant difference in the activity between the treated toxin and the control has been observed. Conclusions: Antibiotics at sub-MIC levels interfere with bacterial biofilm virulence expression depending on the type and concentration of antibiotic used. Significance and Impact of the Study: The establishment of sub-MICs of antibiotics in clinical situations may result in altered virulence states in pathogenic bacteria.
URI: http://hdl.handle.net/10373/461
ISSN: 1364-5072
Appears in Collections:Science Engineering & Technology Collection

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