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Please use this identifier to cite or link to this item: http://hdl.handle.net/10373/462

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Title: Synchronization of yeast cell populations
Authors: Walker, Graeme M.
Affiliation: University of Abertay Dundee. School of Contemporary Sciences
Keywords: Synchronization
Yeast cells
Issue Date: Jun-1999
Publisher: Springer Verlag
Type: Journal Article
Refereed: peer-reviewed
Rights: Published version (c)Springer, available from DOI: 10.1023/A:1009824520278. The original publication is available at www.springerlink.com
Citation: Walker, G. M. 1999. Synchronization of yeast cell populations. Methods in Cell Science. 21(2/3): pp.87-93. Available from DOI: 10.1023/A:1009824520278.
Abstract: The study of synchronous populations of yeast cells has provided a wealth of information into regulatory aspects of the eukaryotic cell division cycle. Synchronized yeast cultures may also have potential benefit when exploiting yeasts in biotechnology. This paper provides an overview of the methods which have been used in the synchronization of cell division in budding and fission yeasts. The relative merits of these methods are outlined and protocols for preferred synchronization methods, based on size selection techniques, are described. In particular, centrifugal elutriation protocols for Saccharomyces cerevisiae (budding yeast) and Schizosaccharomyces pombe (fission yeast) are detailed as this method is regarded as one of the best ways of preparing unperturbed synchronous yeast cultures for cell cycle studies.
URI: http://hdl.handle.net/10373/462
ISSN: 1381-5741
Appears in Collections:Science Engineering & Technology Collection

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