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Title: Anti-Candida activity of a novel killer toxin from the yeast Williopsis mrakii
Authors: Hodgson, Valerie J.
Button, David
Walker, Graeme M.
Affiliation: University of Abertay Dundee. School of Contemporary Sciences
Keywords: Wilhopsis mrakii
Candida spp
Yeast killer toxin
Antifungal activity
Issue Date: 1995
Publisher: Society for General Microbiology
Type: Journal Article
Refereed: peer-reviewed
Rights: Published version (c)Society for General Microbiology, is available from 10.1099/13500872-141-8-2003
Citation: Hodgson, V.J. Button, D. and Walker, G.M. 1995. Anti-Candida activity of a novel killer toxin from the yeast Williopsis mrakii. Microbiology. 141: pp.2003-2012. Available from DOI: 10.1099/13500872-141-8-2003.
Abstract: A screening of putative killer yeast strains showed that spore-forming ascomycetous yeasts of the genera Pichia and Williopsis displayed the broadest range of activity against sensitive strains of Candida spp. and Saccharomyces cerevisiae. Williopsis mrakii (NCYC 500) showed extensive anti-Candida activity against strains isolated from clinical specimens. W. mrakii killer factor was produced in minimal media as a function of growth and its activity reached constant levels as cells entered stationary phase. The proteinaceous killer toxin was found to be unstable outwith a specific range of temperature and pH (above 30 °C and pH 4·0), and further analysis showed that the active toxin molecule was an acidic polypeptide with a relative molecular mass between 1·8-5·0 kDa. At critical concentrations the killer factor exerted a greater effect on stationary phase cells of Candida than cells from an exponential phase of growth. At low concentrations, the killer toxin produced a fungistatic effect on sensitive yeasts but at higher concentrations there was evidence to suggest that membrane damage accounted for the zymocidal effects of the killer factor. The cidal nature of the toxin was reflected in a rapid decrease in sensitive cell viability. Findings presented suggest that W. mrakii killer toxin has potential as a novel antimycotic agent in combatting medically important strains of Candida.
URI: http://hdl.handle.net/10373/465
ISSN: 1350-0872
Appears in Collections:Science Engineering & Technology Collection

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