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|Title: ||A rapid colorimetric assay of killer toxin activity in yeast|
|Authors: ||Hodgson, Valerie J.|
Walker, Graeme M.
|Affiliation: ||University of Abertay Dundee. School of Contemporary Sciences|
|Keywords: ||MTT-colorimetric assay|
|Issue Date: ||1994|
|Type: ||Journal Article|
|Refereed: ||non peer-reviewed|
|Rights: ||Published version (c)Wiley-Blackwell, available from DOI:10.1111/j.1574-6968.1994.tb07031.x|
|Citation: ||Hodgson, V.J., Walker, G.M. and Button, D. 1994. A rapid colorimetric assay of killer toxin activity in yeast. FEMS Microbiology Letters. 120(1/2): pp. 201-205. Available from: doi: 10.1111/j.1574-6968.1994.tb07031.x|
|Abstract: ||The pale yellow redox indicator 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) is reduced to a dark blue end-product, MTT-Formazan, by the mitochondrial dehydrogenases of living cells. MTT reduction can be measured spectrophotometrically at a wavelength of 570 nm and a method is described to assay the cidal activity of Williopsis mrakii killer toxin against sensitive cells of Candida glabrata. The MTT assay is rapid, quantitative and compares favourably with traditional plating techniques for the assessment of sensitive viability.|
|Appears in Collections:||Science Engineering & Technology Collection|
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