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Please use this identifier to cite or link to this item: http://hdl.handle.net/10373/467

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Title: A rapid colorimetric assay of killer toxin activity in yeast
Authors: Hodgson, Valerie J.
Walker, Graeme M.
Button, David
Affiliation: University of Abertay Dundee. School of Contemporary Sciences
Keywords: MTT-colorimetric assay
Killer toxin
Williopsis mrakii
Candicidal activity
Issue Date: 1994
Publisher: Wiley-Blackwell
Type: Journal Article
Refereed: non peer-reviewed
Rights: Published version (c)Wiley-Blackwell, available from DOI:10.1111/j.1574-6968.1994.tb07031.x
Citation: Hodgson, V.J., Walker, G.M. and Button, D. 1994. A rapid colorimetric assay of killer toxin activity in yeast. FEMS Microbiology Letters. 120(1/2): pp. 201-205. Available from: doi: 10.1111/j.1574-6968.1994.tb07031.x
Abstract: The pale yellow redox indicator 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) is reduced to a dark blue end-product, MTT-Formazan, by the mitochondrial dehydrogenases of living cells. MTT reduction can be measured spectrophotometrically at a wavelength of 570 nm and a method is described to assay the cidal activity of Williopsis mrakii killer toxin against sensitive cells of Candida glabrata. The MTT assay is rapid, quantitative and compares favourably with traditional plating techniques for the assessment of sensitive viability.
URI: http://hdl.handle.net/10373/467
ISSN: 0378-1097
Appears in Collections:Science Engineering & Technology Collection

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