Synchronization of cell division in the fission yeast schizosaccharomyces pombe by ethylenediaminetetra-acetic acid
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When cultures of Schizosaccharomyces pombe growing exponentially in a minimal medium were treated with 50 mM-EDTA for 1 h, nucleic acid synthesis was inhibited but protein synthesis was not. On transfer to fresh medium, 50% of the cells divided synchronously. These phenomena may be explained on the basis of reduced availability of Mg2+ following chelation with EDTA.
Ahluwalia, J.H., et al. 1978. Synchronization of cell division in the fission yeast schizosaccharomyces pombe by ethylenediaminetetra-acetic acid. Journal of General Microbiology. 106(2): pp.261-264. [Online] Available from: doi: 10.1099/00221287-106-2-261