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Please use this identifier to cite or link to this item: http://hdl.handle.net/10373/598

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Title: Detection of 8-hydroxy-2'-deoxyguanosine as a marker of oxidative damage in DNA and germplasm exposed to cryogenic treatments
Authors: Johnston, Jason W.
Pimbley, Isobel
Harding, Keith
Benson, Erica E.
Affiliation: University of Abertay Dundee. School of Contemporary Sciences
Keywords: Cryobiology
Free radicals
Oxidative stress
Issue Date: Jan-2010
Publisher: CryoLetters
Type: Journal Article
Refereed: peer-reviewed
Rights: Published version (c)CryoLetters, available from http://www.cryoletters.org/Abstracts/vol_31_1_2010.htm
Citation: Johnston, J.W., et al. 2010. Detection of 8-hydroxy-2'-deoxyguanosine as a marker of oxidative damage in DNA and germplasm exposed to cryogenic treatments. CryoLetters. 31(1): pp.1-13.
Abstract: An HPLC method has been optimised to measure 8-hydroxy-2'-deoxyguanosine (8OHdG) in DNA and germplasm with the objective of using the adduct as a marker of cryostorage stability. The encapsulation-dehydration cryopreservation protocol was adapted as a model system for assessing the formation of 8OHdG from alginate-encapsulated DNA of calf thymus (CT) and currant species (Ribes) exposed to temperatures of -20 and -196ºC. The presence of H2O2 exacerbated the formation of 8OHdG in encapsulated CT and Ribes DNA. Production of the oxidized adduct was lower in the plant system. A reduction in residual water following osmotic dehydration and evaporative desiccation was associated with reduced adduct formation in encapsulated DNA. No significant differences in 8OHdG adduct formation were observed in plants regenerated from cryopreserved Ribes meristems derived from genotypes known to have differential tolerance to cryopreservation.
URI: http://hdl.handle.net/10373/598
ISSN: 0143-2044
Appears in Collections:Science Engineering & Technology Collection

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