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|Title: ||The enhancement of the cellulolytic activity of cellobiohydrolase I and endoglucanase by the addition of cellulose binding domains derived from trichoderma reesei|
|Authors: ||Lemos, Adília|
Teixeira, J. A.
Domingues, M. R. M.
Gama, F. M.
|Affiliation: ||University of Abertay Dundee. School of Contemporary Sciences|
|Keywords: ||Cellulose binding domains|
|Issue Date: ||2003|
|Type: ||Journal Article|
|Rights: ||Published version © Elsevier, available from www.elsevier.com|
|Citation: ||Lemos, A., et.al. 2003. The enhancement of the cellulolytic activity of cellobiohydrolase I and endoglucanase by the addition of cellulose binding domains derived from trichoderma reesei. Enzyme and Microbial Technology. 32(1): pp.35-40. Available from DOI: http://dx.doi.org/10.1016/S0141-0229(02)00235-1|
|Abstract: ||The effect of isolated cellulose binding domains (CBDs) on the hydrolysis of filter paper and microcrystalline cellulose by both cellobiohydrolase I and endoglucanase, was studied. CBDs were obtained by proteolysis from cellulases using a scaled-up variant of our previous method. Experiments were performed for different enzyme/substrate ratios in both the absence and presence of CBDs. Hydrolysis of filter paper by intact cellobiohydrolase I in the presence of additional CBDs was found to have a synergistic effect, leading to an increase of the sugar production of up to 30%. The effect was less pronounced using microcrystalline cellulose, where an increase up to 16% was observed. Similar trends were found during the hydrolysis of both substrates by endoglucanase.|
|Appears in Collections:||Computing & Engineering Systems Collection|
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