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Please use this identifier to cite or link to this item: http://hdl.handle.net/10373/781

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Title: The enhancement of the cellulolytic activity of cellobiohydrolase I and endoglucanase by the addition of cellulose binding domains derived from trichoderma reesei
Authors: Lemos, Adília
Teixeira, J. A.
Domingues, M. R. M.
Mota, M.
Gama, F. M.
Affiliation: University of Abertay Dundee. School of Contemporary Sciences
Keywords: Cellulose binding domains
Synergism
Cellulases
Issue Date: 2003
Publisher: Elsevier
Type: Journal Article
Refereed: peer-reviewed
Rights: Published version © Elsevier, available from www.elsevier.com
Citation: Lemos, A., et.al. 2003. The enhancement of the cellulolytic activity of cellobiohydrolase I and endoglucanase by the addition of cellulose binding domains derived from trichoderma reesei. Enzyme and Microbial Technology. 32(1): pp.35-40. Available from DOI: http://dx.doi.org/10.1016/S0141-0229(02)00235-1
Abstract: The effect of isolated cellulose binding domains (CBDs) on the hydrolysis of filter paper and microcrystalline cellulose by both cellobiohydrolase I and endoglucanase, was studied. CBDs were obtained by proteolysis from cellulases using a scaled-up variant of our previous method. Experiments were performed for different enzyme/substrate ratios in both the absence and presence of CBDs. Hydrolysis of filter paper by intact cellobiohydrolase I in the presence of additional CBDs was found to have a synergistic effect, leading to an increase of the sugar production of up to 30%. The effect was less pronounced using microcrystalline cellulose, where an increase up to 16% was observed. Similar trends were found during the hydrolysis of both substrates by endoglucanase.
URI: http://hdl.handle.net/10373/781
ISSN: 0141-0229
Appears in Collections:Computing & Engineering Systems Collection

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