Logo
 

Abertay Research Collections >
Research Centres >
SIMBIOS Collection >

Please use this identifier to cite or link to this item: http://hdl.handle.net/10373/786

View Statistics
Title: In situ visualisation of fungi in soil thin sections: problems with crystallisation of the fluorochrome FB 28 (Calcofluor M2R) and improved staining by SCRI Renaissance 2200
Authors: Harris, Kirsty
Crabb, David
Young, Iain M.
Weaver, Howard
Gilligan, Christopher A.
Otten, Wilfred
Ritz, Karl
Affiliation: University of Abertay Dundee. Scottish Informatics, Mathematics, Biology and Statistics Centre
Keywords: Soils
Fungi
Issue Date: Mar-2002
Publisher: Elsevier
Type: Journal Article
Refereed: peer-reviewed
Rights: Published version (c)Elsevier, available from DOI: 10.1017/S0953756202005749
Citation: Harris, K., et al. 2002. In situ visualisation of fungi in soil thin sections: problems with crystallisation of the fluorochrome FB 28 (Calcofluor M2R) and improved staining by SCRI Renaissance 2200. Mycological Research. 106(3): pp.293-297. Available from http://dx.doi.org/10.1017/S0953756202005749
Abstract: Fluorescent stains offer an effective means of visualising bacteria and fungi in soil or litter samples. Fluorescent brightener (FB) 28 (also known as Calcofluor White M2R) is commonly used to stain such microorganisms. However, during production of soil thin-sections we observed erratic crystallisation of this stain, particularly in soils colonised by Rhizoctonia solani. We report on the evaluation of alternative stains to FB 28 for their propensity to crystallize in interaction with fungi, staining efficiency, and suitability for application in soil thin-section production. All of the additional candidate stains namely Fluorescent Brightener Agent (FBA) 15/25, FBA 71 and SCRI Renaissance 2200 (SR 2200) were highly effective in staining agar-cultured hyphae, but differed in the degree to which they stained hyphae cultured in soil. All stains tested, except SR 2200, stained hyphae of R. solani insufficiently when growing on or through soil. These stains also showed extensive crystallisation in solutions that had been in contact with R. solani colonised soil. However, SR 2200 stained hyphae of R solani growing over soil as effectively as hyphae growing on agar and showed no evidence of crystallisation; the intensity of staining exceeded that of the ‘benchmark’ FB 28 for hyphae grown in two soil types. These excellent fluorescent properties of FBA 220 persisted in soil thin-sections, resulting in bright hyphae that could be readily visualised in situ in undisturbed soils.
URI: http://hdl.handle.net/10373/786
ISSN: 0953-7562
Appears in Collections:SIMBIOS Collection
Science Engineering & Technology Collection

Files in This Item:

There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Valid XHTML 1.0! DSpace Software Copyright © 2002-2010  Duraspace - Feedback