Theoretical mechanisms in targeted and random integration of transgene DNA
The genetic manipulation of mammalian cells and animals would be greatly expedited if gene targeting could be reliably achieved in the widest possible range of host cell types. This paper considers empirical evidence and theoretical considerations associated with transgene integration, and concludes that utilisation of gene targeting in non-selective systems awaits further progress in modelling homologous recombination.
Smith, K.R. 2001. Theoretical mechanisms in targeted and random integration of transgene DNA. Reproduction Nutrition Development. 41(6): pp.465-485. Available from http://dx.doi.org/10.1051/rnd:2001102